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A dialysis membrane is a semi-permeable film (usually a sheet of regeneratedcellulose) containing various sized pores. Molecules larger than the pores cannot passthrough the membrane but small molecules can do so freely. In this manner, dialysis maybe used to perform purification or buffer exchange for samples containingmacromolecules. The time required to accomplish dialysis is determined by factors thataffect the rate of diffusion of a molecule. Because heat affects the thermodynamics ofmolecules, increasing temperature diffusion speeds. Therefore, dialysis will proceedfaster at room temperature than at 4° c. The rate of diffusion is also directly proportionalto the concentration hand of a molecule, while inversely proportional to its molecular weight.As the concentration hand of a molecule increases, so does the probability that one of those
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