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As a result of a successful crosslinking glutaraldehyde agent is itsThe multicomponent nature is present in several forms at the equilibrium in a given pH value in the reagent solution. However, the accurate solution of the glutaraldehyde molecular composition, as well as theThis is the most part, is debatable despite the knowledge. As a result, the reaction mechanism with the protein of the amino glutaraldehyde group is not clearly understood,In order to illustrate the reported by a large number of mechanisms, the literature is reviewed and summarized in this. No single mechanism seems to be responsible for crosslinking glutaraldehydeAnd protein. It is reported that all forms of reaction and the ability to exhibit the ability of crosslink glutaraldehyde to form a large, leading wide range of conjugates. A rather hard controlReaction conditions are required to achieveEach different valid insolubilizationBecause of the structure of the enzymeVariability. Nonetheless, resultingEnzyme derivatives general programSo, you can reused and very good stability.Although the inactivation bias of the enzymeIs often due to chemical modificationIn most cases, enough unavoidable,Catalytic activity is retained. More workIt is necessary to provide additional informationIn the accuracy of these structuresCrosslinked products.
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