Sau 6 tuần nuôi cấy, các bộ phận lá

Sau 6 tuần nuôi cấy, các bộ phận lá, thân, rễ ở Spilanthes calva có tỷ lệ cảm ứng mô sẹo khác nhau đối với các nồng độ 2,4-D và kinetin khác nhau, cao nhất là 100% và thấp nhất là 33%, tất cả sẹo được cảm ứng thành công đều là sẹo xốp. Các bộ phận cây có dấu hiệu bắt đầu hình thafh mô sẹo từ sau khoảng 3-4 tuần và hoàn toàn chuyển hóa thành mô sẹo ở cuối tuần thứ 6. Cô sẹo cảm ứng từ lá cho màu xanh lá sáng, hơi ngả vàng, trong khi các mô sẹo được cảm ứng từ rê và thân lại có màu xanh lá hơi tối. Ở lá, mô sẹo được cảm ứng có màu xanh lá hơi ngả vàng, khối lượng tươi và khô cao nhất được thu nhận ở nghiệm thức 0.5 mg/mL 2,4-D với tỷ lệ cảm ứng là 100%, ở các nghiệm thức chỉ cảm ứng bằng kinetin thì không ghi nhận sự xuất hiện của mô sẹo. Ở thân, mô sẹo được cảm ứng có màu xanh lá đậm, khối lượng tươi và khối lượng khô cao nhất được thu nhận lần lượt ở nghiệm thức 0.5 mg/mL 2,4-D và 2,4-D kết hợp với kinetin (1x1 mg/mL). Ở rễ, mô sẹo được cảm ứng có màu xanh lá đậm và ở một số nghiệm thức có màu xanh đậm hơn cả mô sẹo thân, khối lượng tươi và khối lượng khô cao nhất được thu nhận lần lượt ở nghiệm thức 2,4-D kết hợp với kinetin (0.5 x 3 mg/mL) và 2 mg/mL 2,4-D.

Results (English) 1:

After 6 weeks of culture, the leaf, stem, and root parts of Spilanthes calva had different callus induction rates for different concentrations of 2,4-D and kinetin, the highest being 100% and the lowest being 100%. In 33%, all scars that were successfully induced were spongy scars. The plant parts show signs of starting to form callus after about 3-4 weeks and completely transform into callus at the end of the 6th week. The callus from the leaves gives a bright green, slightly yellowish color. while the callus induced by mulberry and stem has a slightly dark green color. In the leaves, the induced callus had a slightly yellowish green color, the highest fresh and dry weight was obtained in the 0.5 mg/mL 2,4-D treatment with an induction rate of 100%, in the treatments. Induction with only kinetin did not show the appearance of scar tissue. In the stem, the induced callus had a dark green color, the highest fresh weight and dry weight were obtained in the 0.5 mg/mL 2,4-D and 2,4-D combined with kinetin treatments, respectively. (1x1 mg/mL). In the roots, the induced callus was dark green and in some treatments darker green than the stem callus. The highest fresh weight and dry weight were obtained in the 2.4- treatment, respectively. D combined with kinetin (0.5 x 3 mg/mL) and 2 mg/mL 2,4-D.

Results (English) 2:

After 6 weeks of cultivation, the leaves, stems, and roots of Spilanthes calva showed different scar tissue induction rates for different concentrations of 2.4-D and actin, with the highest being 100% and the lowest being 33%. All successfully induced scars were porous scars. The plant part begins to form scar tissue approximately 3-4 weeks later and completely transforms into scar tissue by the end of week 6. Her leaves induce scars that appear light green and slightly yellow, while the scar tissue induced by the roots and stems appears dark green. In the leaves, the induced scar tissue was slightly yellow, and the highest fresh and dry quality was obtained at an induction rate of 100% at 0.5mg/mL 2.4-D and 0.5mg/mL 2.4-D. The appearance of scar tissue was not recorded in experiments induced solely with creatinine. In the trunk, the induced scar tissue was dark green, and the highest fresh and dry masses were obtained in the combination of 0.5mg/mL 2.4-D and 2.4-D with creatinine (1x1mg/mL), respectively. In the root, the induced scar tissue appears dark green, and in some experiments

Results (English) 3:

After six weeks of culture, leaves, bodies and roots of Spilanthes calva have different scar tissue sensitivity rates to different concentrations of 2,4-D and hormones, with the highest being 100% and the lowest being 33%. All the scars successfully induced are spongy scars. There are signs in some parts of the tree that scar tissue began to form after about 3-4 weeks, and completely transformed into scar tissue at the end of 6 weeks. Scar is sensed from leaves to light green, which turns slightly yellow, while scar tissue sensed from curly hair and trunk presents a slightly dull green color. In the leaves, the induced scar tissue turns slightly yellow and green, and the highest fresh and dry quality is received when identifying 0. 5 mg/ml 2,4-D, the induction rate was 100%, and no scar tissue was observed in the cognition only using hormone induction. In vivo, the induced scar tissue is dark green, and the fresh volume and the highest dry volume are received when identifying 0 respectively. 5 mg/ml of 2,4-D and 2,4-D combined agonist (1x1 mg/ml). At the root, scar tissue is perceived as dark green, and in some senses, blue is deeper than body scar tissue, and the highest fresh and dry amount is obtained in the perception of 2,4-D binding hormone (0) respectively. 5×3 mg/ml) and 2 mg/ml 2,4-d.