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對於所有實驗,樣品分別從真空包裝中取出,無菌地轉移到胃部®400個經典袋(目錄號。BA6041, 斯圖爾特有限公司, 沃辛, 西蘇塞克斯, 英國) 包含 30 mL 的戴英利中和肉湯 (目錄號C7371,哈迪診斷,聖瑪麗亞,加利福尼亞州,美國),然後混合30 s在250 rpm(胃®400循環器,管家有限公司,沃辛,西蘇塞克斯,英國)。對於所有樣品,從無菌BPD的初始稀釋中製備了10倍稀釋液。每次稀釋都表面鍍到CLA上,然後以42°C微航空嗜血症進行48小時的孵育。對於有氧細菌枚舉,每個稀釋被鍍在試吃大豆agar上(TSA;目錄代碼DF0369176、貝克頓、迪金森和公司、Sparks、MD、美國)隨後在24小時有氧條件下在37°C下孵育。細菌菌落被計數並表示為cfu/樣本。 ...

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